糖心logo入口

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CATEGORIES:College of Arts and Sciences,Thesis/Dissertations
DESCRIPTION:Advisor: Dr. Mark Silby mark.silby@umassd.edu Committee: Dr. Ch
 ristopher Brigham, Dr. Robert Drew Abstract: Rhizobacteria such as Pseudom
 onas fluorescens Pf0-1 are important contributors to plant health due to t
 heir involvement in nutrient uptake, regulation of plant immunity, and inh
 ibition of pathogens. P. fluorescens Pf0-1 mediates movement by using micr
 oscopic hair-like structures called flagella. Flagella synthesis is contro
 lled by the bacterial enhancer binding protein (bEBP) FleQ; fleQ mutants a
 re unable to swim. Taylor et al., (2015) showed that fleQ mutants eventual
 ly regain motility due to new mutations in ntrB and ntrC which result in e
 levated activation of NtrC and off-target binding of the bEBP NtrC to FleQ
 -controlled promoters. We hypothesized that if mutations that activate bEB
 Ps can lead to crosstalk between regulatory networks, environmental signal
 s that activate bEBPs may also influence crosstalk in a post-translational
  manner. We tested whether glutamate or alpha ketoglutarate which are pred
 icted to activate AauR and MifR respectively could restore motility in the
  Pf0-1 fleQ mutant and found that the addition of glutamate restored motil
 ity. However, deletion of aauR did not abolish motility restoration, indic
 ating that a different bEBP was important. Surprisingly, deletion of ntrC 
 rendered glutamate unable to promote motility restoration, demonstrating t
 hat in the presence of glutamate wildtype NtrC can functionally replace Fl
 eQ in P. fluorescens. Measurement of transcription of the gene flhA which 
 is normally FleQ-controlled confirmed that in the presence of glutamate, N
 trC is capable of regulating expression of flagella genes. This research l
 ooks to provide an insight on how crosstalk between different regulatory p
 athways can increase the adaptability of bacteria in complex natural envir
 onments.\nEvent page: /events/cms/biology-master-the
 sis-defense-by-sharon-jacob.php
X-ALT-DESC;FMTTYPE=text/html:<html><Body><h1><a href="/">糖心logo入口</a></h1><script>
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</script><p>Advisor: Dr. Mark Silby mark.si
 lby@umassd.edu</p>\n<p>Committee: Dr. Christopher Brigham\, Dr. Robert Dre
 w</p>\n<p>Abstract: Rhizobacteria such as Pseudomonas fluorescens Pf0-1 ar
 e important contributors to plant health due to their involvement in nutri
 ent uptake\, regulation of plant immunity\, and inhibition of pathogens. P
 . fluorescens Pf0-1 mediates movement by using microscopic hair-like struc
 tures called flagella. Flagella synthesis is controlled by the bacterial e
 nhancer binding protein (bEBP) FleQ\; fleQ mutants are unable to swim. Tay
 lor et al.\, (2015) showed that fleQ mutants eventually regain motility du
 e to new mutations in ntrB and ntrC which result in elevated activation of
  NtrC and off-target binding of the bEBP NtrC to FleQ-controlled promoters
 . We hypothesized that if mutations that activate bEBPs can lead to crosst
 alk between regulatory networks\, environmental signals that activate bEBP
 s may also influence crosstalk in a post-translational manner. We tested w
 hether glutamate or alpha ketoglutarate which are predicted to activate Aa
 uR and MifR respectively could restore motility in the Pf0-1 fleQ mutant a
 nd found that the addition of glutamate restored motility. However\, delet
 ion of aauR did not abolish motility restoration\, indicating that a diffe
 rent bEBP was important. Surprisingly\, deletion of ntrC rendered glutamat
 e unable to promote motility restoration\, demonstrating that in the prese
 nce of glutamate wildtype NtrC can functionally replace FleQ in P. fluores
 cens. Measurement of transcription of the gene flhA which is normally FleQ
 -controlled confirmed that in the presence of glutamate\, NtrC is capable 
 of regulating expression of flagella genes. This research looks to provide
  an insight on how crosstalk between different regulatory pathways can inc
 rease the adaptability of bacteria in complex natural environments.</p><p>
 Event page: <a href="/events/cms/biology-master-thes
 is-defense-by-sharon-jacob.php">/events/cms/biology-
 master-thesis-defense-by-sharon-jacob.php</a></a></p></body></html>
DTSTAMP:20260525T052920
DTSTART;TZID=America/New_York:20260610T100000
DTEND;TZID=America/New_York:20260610T110000
LOCATION:LIB-314
SUMMARY;LANGUAGE=en-us:Biology Master Thesis Defense by Sharon Jacob
UID:d5fbc18dc6ac4bc22dac33704419cf41@www.umassd.edu
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