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CATEGORIES:College of Arts and Sciences,Lectures and Seminars,Thesis/Disser
 tations
DESCRIPTION:Title:Ā  Cranberry Leaf Extracts as a Tool Against Staphylcoccu
 s aureus: Composition and Biofilm Inhibitory Properties by Kyla Lockhart A
 dvisor:Ā Ā Dr. Catherine Neto,Ā Chemistry & Biochemistry Dept. Ā Committee
  Members:Ā Ā Dr. Shuowei Cai,Ā Chemistry & Biochemistry Dept. & Dr. Frank 
 Scarano, Medical Labratory Science Abstract:Ā Ā Biofilm is a community of 
 micro-organisms which forms as a means of protection. This community helps
  to protect the bacteria from its environment and to adhere to different s
 urfaces. Staphylococcus aureus is a common gram-positive bacterial pathoge
 n bacteria which forms biofilm by producing extracellular polysaccharide (
 EPS). This process allows these bacteria to spread and become harder to ki
 ll as the EPS makes it more resistant to antibiotics. Cranberries are know
 n for their antibacterial effects due to the proanthocyanidins (PACs) they
  contain. While there has been much research on the cranberry fruit, there
  is less known about the chemistry and antibacterial properties of the cra
 nberry leaves. Through bioassay-guided fractionation of leaf extracts usin
 g Sephadex-LH20 chromatography and LCMS and GCMS analysis we were able to 
 characterize cranberry leaf fractions and observe their anti-biofilm effec
 ts on S. aureus. In the biofilm formation and eradication assays, fraction
  EDII was the most effective, with inhibition of 88% and 78% respectively 
 at a concentration of 12.5 Āµg/mL. EDII was found to contain isomers of th
 e phenolic compound p-coumaroylquinic acid and was further separated into 
 subfractions which were analyzed through LCMS and biological testing. The 
 best result for biofilm inhibition was obtained for fraction F5 with 83% a
 nd the best biofilm eradication was obtained for fraction F4 with 68%. The
 se fractions were found to contain 1-p-coumaroylquinic acid, 5-p-coumaroyl
 quinic acid and chicoric acid which may contribute to their anti-bacterial
  and antioxidant effects. ED fractions and subfractions were also tested f
 or antioxidant activity using the DPPH assay. Many of these fractions had 
 promising free radical scavenging activity, with the the most promising re
 sults observed for fractions EDVI and EDVII and subfraction F5 which had I
 C50 values of 3.64, 1.21, and 17.7 Āµg/mL respectively. Overall, these fin
 dings demonstrate that cranberry leaves contain strong anti-biofilm and an
 tioxidant properties which suggests they have potential as a natural antim
 icrobial source and sets the foundation for future studies in isolation an
 d characterization of active compounds and their activity.\nEvent page: ht
 tps://www.umassd.edu/events/cms/20260721-ms-thesis-defense-by-kyla-lockhar
 t.php
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</script><p>Title:Ā  Cranberry Leaf Extract
 s as a Tool Against Staphylcoccus aureus: Composition and Biofilm Inhibito
 ry Properties by Kyla Lockhart</p>\n<p>Advisor:Ā Ā Dr. Catherine Neto\,Ā C
 hemistry & Biochemistry Dept.</p>\n<p>Ā Committee Members:Ā Ā Dr. Shuowei 
 Cai\,Ā Chemistry & Biochemistry Dept. & Dr. Frank Scarano\, Medical Labrat
 ory Science</p>\n<p>Abstract:Ā Ā Biofilm is a community of micro-organisms
  which forms as a means of protection. This community helps to protect the
  bacteria from its environment and to adhere to different surfaces. Staphy
 lococcus aureus is a common gram-positive bacterial pathogen bacteria whic
 h forms biofilm by producing extracellular polysaccharide (EPS). This proc
 ess allows these bacteria to spread and become harder to kill as the EPS m
 akes it more resistant to antibiotics. Cranberries are known for their ant
 ibacterial effects due to the proanthocyanidins (PACs) they contain. While
  there has been much research on the cranberry fruit\, there is less known
  about the chemistry and antibacterial properties of the cranberry leaves.
  Through bioassay-guided fractionation of leaf extracts using Sephadex-LH2
 0 chromatography and LCMS and GCMS analysis we were able to characterize c
 ranberry leaf fractions and observe their anti-biofilm effects on S. aureu
 s. In the biofilm formation and eradication assays\, fraction EDII was the
  most effective\, with inhibition of 88% and 78% respectively at a concent
 ration of 12.5 Āµg/mL. EDII was found to contain isomers of the phenolic c
 ompound p-coumaroylquinic acid and was further separated into subfractions
  which were analyzed through LCMS and biological testing. The best result 
 for biofilm inhibition was obtained for fraction F5 with 83% and the best 
 biofilm eradication was obtained for fraction F4 with 68%. These fractions
  were found to contain 1-p-coumaroylquinic acid\, 5-p-coumaroylquinic acid
  and chicoric acid which may contribute to their anti-bacterial and antiox
 idant effects. ED fractions and subfractions were also tested for antioxid
 ant activity using the DPPH assay. Many of these fractions had promising f
 ree radical scavenging activity\, with the the most promising results obse
 rved for fractions EDVI and EDVII and subfraction F5 which had IC50 values
  of 3.64\, 1.21\, and 17.7 Āµg/mL respectively. Overall\, these findings d
 emonstrate that cranberry leaves contain strong anti-biofilm and antioxida
 nt properties which suggests they have potential as a natural antimicrobia
 l source and sets the foundation for future studies in isolation and chara
 cterization of active compounds and their activity.</p><p>Event page: <a h
 ref="/events/cms/20260721-ms-thesis-defense-by-kyla-
 lockhart.php">/events/cms/20260721-ms-thesis-defense
 -by-kyla-lockhart.php</a></a></p></body></html>
DTSTAMP:20260717T023549
DTSTART;TZID=America/New_York:20260721T130000
DTEND;TZID=America/New_York:20260721T150000
LOCATION:SENG 307B
SUMMARY;LANGUAGE=en-us:MS Thesis Defense by Kyla Lockhart, Cranberry Leaf E
 xtracts as a Tool Against Staphylcoccus aureus: Composition and Biofilm In
 hibitory Properties
UID:b56d0ff25d68dc16cb802c0e6ddcea62@www.umassd.edu
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